It is well know like the most common causative agents of diarrhea in young calves are, among others:
Enteropathogenic or enterotoxic Escherichia coli (K99, K88, etc.)
Salmonella species
Clostridium perfringens type C
Cryptosporidium
Eimeria species
Nematodes such as Strongyloides Rotavirus
Coronavirus
Other viruses
Well, after acquiring a direct ELISA kit for diarrhea in calves (Cypress Diagnostics), my friend the engineer Luis Gonzalez and I dedicated to the task of searching and processing stool samples of young calves with diarrhea to demonstrate presence of “bugs” Kit includes: E coli K99, rotavirus, coronavirus and Cryptosporidium. In addition, the samples were practicing the Willis flotation method for light eggs as Eimeria and nematodes. It was also used in coloring of feces by the method Ziehl Neelsen for Cryptosporidium modified.
The big surprise is that we get a lot more calves with Cryptosporidium as other better know for us disease agents. The kit worked well, as indicated by positive and negative controls and the optical densities obtained, but that was the result.
Important to be noted that bacteriology cultures was not performed in the stool and also the strain of E coli tested was K99. There are reports in cattle indicate the importance of K88, which in this case was not tested. However, it shows the importance of Cryptosdporidium agent that is often not taken into account in Venezuela.
The following link leads to a summary of this initial work:
Enteropathogenic or enterotoxic Escherichia coli (K99, K88, etc.)
Salmonella species
Clostridium perfringens type C
Cryptosporidium
Eimeria species
Nematodes such as Strongyloides Rotavirus
Coronavirus
Other viruses
Well, after acquiring a direct ELISA kit for diarrhea in calves (Cypress Diagnostics), my friend the engineer Luis Gonzalez and I dedicated to the task of searching and processing stool samples of young calves with diarrhea to demonstrate presence of “bugs” Kit includes: E coli K99, rotavirus, coronavirus and Cryptosporidium. In addition, the samples were practicing the Willis flotation method for light eggs as Eimeria and nematodes. It was also used in coloring of feces by the method Ziehl Neelsen for Cryptosporidium modified.
The big surprise is that we get a lot more calves with Cryptosporidium as other better know for us disease agents. The kit worked well, as indicated by positive and negative controls and the optical densities obtained, but that was the result.
Important to be noted that bacteriology cultures was not performed in the stool and also the strain of E coli tested was K99. There are reports in cattle indicate the importance of K88, which in this case was not tested. However, it shows the importance of Cryptosdporidium agent that is often not taken into account in Venezuela.
The following link leads to a summary of this initial work:
http://investigacion.unet.edu.ve/lifsa/pdf/GonzalezMoreno.pdf
After this, we have been investigating the true significance of Cryptosporidium as a cause of clinical cases of diarrhea in calves, which is reason of later publications that I will comment later.
Also important is the zoonotic nature of cryptosporidiosis, as it can affect humans, especially children and people with some degree of immunosuppression.
The water may be still contaminated by these feces and other farm or wild species feces and Cryptosporidium may be polluting the drinking water sources of our cities, taking into account that the chlorine treatment is not effective against this protozoan.
After this, we have been investigating the true significance of Cryptosporidium as a cause of clinical cases of diarrhea in calves, which is reason of later publications that I will comment later.
Also important is the zoonotic nature of cryptosporidiosis, as it can affect humans, especially children and people with some degree of immunosuppression.
The water may be still contaminated by these feces and other farm or wild species feces and Cryptosporidium may be polluting the drinking water sources of our cities, taking into account that the chlorine treatment is not effective against this protozoan.
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